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c9214 [9214]

規格:

價格:

編號:B237588

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

c9214 [9214]

商品貨號

B237588

Designations

c9214 [9214]

Species

Saccharomyces cerevisiae Meyen ex E.C. Hansen

培養基

Depositors

MV Olson, L Riles

Vector

Construct size (kb): 0.0

Insert

DNA: genomic

Gene product: 3-isopropylmalate dehydrogenase(beta-isopropylmalate dehydrogenase) [LEU2]

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Related Products

component of:ATCC 77284

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.399, 2.265, 4.747, 1.202, 1.816, 4.232, 2.260, 1.414, 3.064, 3.478, 3.682.

Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 1.2, 1.4, 1.9, 2.3, 3.2, 3.7, 3.8, 4.3, 5.0.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Overlaps clones with ATCC number(s): 70303, 70648, 70649.

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

References

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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