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YEp24 [pRB5]

規(guī)格:

價格:

編號:B237719

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

YEp24 [pRB5]

商品貨號

B237719

Designations

YEp24 [pRB5]

Depositors

D Botstein

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 7.7690000534057620

Vector: YEp24 (plasmid)

Promoters: Promoter none

Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori

Marker(s):URA3,ampR,tetR

Construct size (kb): 7.769000053405762

Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none

Applications

YE-type (episomal) shuttle vector

shuttle vector

vector for constructing genomic libraries

Comments

YE-type episomal shuttle vector.

Plasmid was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites of pBR322.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863