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pWS12

規格:

價格:

編號:B237754

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pWS12

商品貨號

B237754

Designations

pWS12

GenBank Number

M11133

培養基

Species

Wound tumor virus

Depositors

DL Nuss

Vector

Construct size (kb): 3.72000002861023

Insert

DNA: cDNA

Insert lengths(kb): 0.8500000238418579

Gene product: segment S12, Pns12 polypeptide

Insert Size (kb)

0.850

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains internal HindIII and AccI sites.

Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.90; EcoRI--3.8; PstI--3.8; HindIII--3.0, 0.87; XbaI--3.8.

Linearize the plasmid with AvaI for in vitro transcription.

This is a full-length clone of virus segment S12.

The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.

Classification

Phytoreovirus

References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

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