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HFBCD05

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價格:

編號:B237759

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

HFBCD05

商品貨號

B237759

Designations

HFBCD05

GenBank Number

M77924

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.699999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DLAT
Other gene symbols: DLTA
Gene name: dihydrolipoamide S-acetyltransferase (E2 component of
pyruvate dehydrogenase complex)
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q23.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M77924; EST: EST01508; Primer: M13 Forward;
THC id: THC121801

Insert lengths(kb): 1.700000047683716

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)( M2 antigen complex 70 kDa subunit, dihydrolipoamide S-acetyltransferase) [DLAT]

Insert Size (kb)

1.700

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.8; BamHI--4.7; PstI--3.8, 0.7, 0.2.

EST01508 matches GenBank accession HUMPDCE2 with 98.43% identity over the sequence range: 1-255.

References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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