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pPA8

規格:

價格:

編號:B237837

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pPA8

商品貨號

B237837

Designations

pPA8

GenBank Number

X07653

培養基

Species

Barley yellow dwarf luteovirus

Depositors

WA Miller

Vector

Construct size (kb): 4.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 1.700000047683716

Gene product: coat protein

Insert Size (kb)

1.700

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert includes the following restriction sites (with the PstI site adjacent to the HindIII site of the polylinker = 1): AvaI--151; BamHI--187; PvuII--620; AvaI--711; KpnI--870; EcoRI--876; AccI--1563; EcoRI--1683.

Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.7; EcoRI--2.9, 0.8, 0.7; HindIII--4.4.

The insert is oriented with the 5' side (3308) closer to the EcoRI site of the polylinker.

The insert includes nucleotides 3308 through 5011, encoding part of the coat protein and the overlapping 17K ORF, all of the 50K ORF, and part of the 6.7K ORF.

Classification

Luteovirus

References

Miller WA, et al. Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Res. 16: 6097-6111, 1988. PubMed: 3399386

W A Miller, personal communication

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