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BDCM
BDCM
規格:
價格:
編號:B237868
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BDCM
商品貨號
B237868
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
B lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain EBV viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute myelogenous leukemia (AML)
Age
unknown
Gender
male
Ethnicity
unknown
Applications
Cells may be useful in modeling immune reactions.
Storage Conditions
liquid nitrogen vapor phase
Derivation
BDCM is a B cell line established from a pheresis sample from a patient with M5a leukemia.
Clinical Data
male
unknown
Antigen Expression
CD20; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD40; Homo sapiens, expressed
CD80; Homo sapiens, expressed
CD83; Homo sapiens, expressed
CD86; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
CD1a; Homo sapiens, not expressed
CD11c; Homo sapiens, not expressed
Receptor Expression
interleukin 3 (IL-3)
Comments

In addition to B cell markers, the cells have many characteristics of dendritic cells.

The cells are positive for Epstein-barr virus (EBV).

They produce fascin, interleukin 1 alpha (IL-1 alpha), interleukin 2 (IL-2), interleukin 6 (IL-6), interleukin 16 (IL-16), transforming growth factor beta1 (TGF beta1), and tumor necrosis factor (TNF).

 

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 cells/mL and maintain between 3 X 105 and 3 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 10,11
D5S818: 12,13
D7S820: 9,11
THO1: 6,9.3
TPOX: 9,10
vWA: 16,17
Name of Depositor
RA Steinman
Deposited As
human
References

Kharbanda S, et al. BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells. Br. J. Haematol. 119: 819-825, 2002. PubMed: 12437665

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