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pG143/T2

規(guī)格:

價格:

編號:B237889

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pG143/T2

商品貨號

B237889

Designations

pG143/T2

Species

Saccharomyces cerevisiae Meyen ex E.C. Hansen

培養(yǎng)基

Depositors

A Tzagoloff

Vector

Construct size (kb): 17.5

Insert

DNA: genomic

Insert lengths(kb): 9.300000190734863

Gene product: transcription activation factor HAP3 [HAP3]

Insert Size (kb)

9.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains the following sites separated by (approximate kb): EcoRI- 2.2- XbaI- 0.6- SstI- 0.7- EcoRI- 0.9- BamHI- 0.25- SphI- 0.55- SstI- 0.1- SphI- 0.7- XbaI.

Restriction digests of the clone give the following sizes (kb): 12.0, 4.65, 1.0; BamHI--17.5; SmaI/SalI--7.6 (doublet), 2.25; KpnI--uncut.

The HAP3 gene is included in a 1.6 kb BamHI/XbaI fragment.

The HAP3 locus encodes 2 overlapping transcripts of 0.57 kb and 4.0 kb read from opposite strands. The smaller mRNA, encoding a protein of 144 aa, directs the synthesis of a protein required for UAS2 activity.

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

References

Hahn S, et al. The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts. Mol. Cell. Biol. 8: 655-663, 1988. PubMed: 2832732

Alexander Tzagoloff, personal communication

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