Comments
Restriction digests of the clone give the following sizes (kb): AvaII--2.8, 0.25; BamHI--3.1; BglI--3.1; DraI--2.4, 0.74; EcoRI--2.8, 0.28; PstI--3.1.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.03, HaeIII--0.15.
A unique sequence probe corresponding to nt 1066-1291 of the published sequence for form 3 of the receptor.
Transcription from the SP6 promoter gives the sense strand (linearize with HindIII. Transcription from the T7 promoter gives the anisense strand (linearze with BamHI).