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clone 13
clone 13
規格:
價格:
編號:B238737
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
clone 13
商品貨號
B238737
Designations
clone 13
GenBank Number

X68274

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 7.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TAX
Other gene symbols: TAG1
Genomic copy number: unique
Gene name: transiently-expressed axonal glycoprotein (axonin-1)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 q32
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.5
Cross references: DNA Seq. Acc.: X68274
Insert lengths(kb): 4.5
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: axonal glycoprotein TAG-1( axonal glycoprotein TAG-1, axonin-1 cell adhesion molecule, transiently-expressed axonal glycoprotein, transiently-expressed axonal glycoprotein (axonin-1)) [TAX]
Insert Size (kb)
4.5
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.0, 0.6; HindIII/XbaI--4.5, 2.95; BamHI--4.3, 3.0, 0.2; PstI--3.15, 1.1 (doublet), 0.9, 0.6, 0.5; KpnI--3.3, 3.25, 1.0.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.26, 3.15; BglI--0.02, 1.15, 1.84, 2.26, 3.37, 3.86; EcoRI--0.57; KpnI--0.40, 1.38; PstI--0.57, 1.05, 2.12, 3.00, 3.16, 4.23, 4.25;
PvuII--0.11, 0.44, 1.03, 1.91, 2.05, 2.60, 2.75, 3.32; SacI--2.35, 3.29, 3.55, 3.78; SmaI--0.36, 1.50, 2.46; XhoI--3.80.
Contains the complete coding sequence, starting at the 5' end of the published sequence, with an additional T at position 2986, responsible for a frame shift and early stop codon.
The GenBank record and reference show the corrected sequence.
References

Hasler TH, et al. cDNA cloning, structural features, and eukaryotic expression of human TAG-1/axonin-1. Eur. J. Biochem. 211: 329-339, 1993. PubMed: 8425542

Kenwrick S, et al. Localization of the human TAX-1 gene to 1q32.1: a region implicated in microcephaly and Van der Woude syndrome. Hum. Mol. Genet. 2: 1461-1462, 1993. PubMed: 8242070

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