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BHK570
BHK570
規格:
價格:
編號:B238774
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BHK570
商品貨號
B238774
Organism
Mesocricetus auratus, hamster, Syrian golden
Tissue
kidney
培養基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Used in producing a mammalian G protein coupled glutamate receptor [U.S. Pat. 5,385,831].
The cells are useful as hosts for transfection studies.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Comments
The BHK-570 cell line is a baby hamster kidney cell line that is thymidine kinase deficient (TK-) RefHorn IR, et al. Molecular analysis of ligant binding to the second cluster of complement-type repeats of the low density lipoprotein receptor-related protein. J. Biol. Chem. 272: 13608-13613, 1997. PubMed: 9153209. The cells are useful as hosts for transfection studies. Used in producing a mammalian G protein coupled glutamate receptor [U.S. Pat. 5,385,831].
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
Univ. Washington, ZymoGenetics, Inc.
Deposited As
hamster
U.S. Patent Number
References

Mulvihill ER, et al. Method for producing a mammalian G protein copuled glutamate receptor. US Patent 5,385,831 dated Jan 31 1995

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