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pCEA1
pCEA1
規(guī)格:
價(jià)格:
編號(hào):B239487
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pCEA1
商品貨號(hào)
B239487
Designations
pCEA1
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.8299999833106995
Tissue: colon adenocarcinoma
Gene product: carcinoembryonic antigen [CEA]
Insert Size (kb)
0.830
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.54 + others; EcoRI--5.3; HindIII--5.3; BamHI--5.3; SalI--5.3.
The insert contains HinfI, HpaII, PstI, HincII, SstI, and PstI sites. The PstI sites at the ends were created by the cloning procedure.
This is a 5' end probe for CEA.
Open reading frame encoded in pCEA1 and pCEA2 contains 2 closely-related repeats of 178 amino acids each. pCEA1 contains repeat a and part of repeat b; pCEA2 contains repeat b and part of repeat a.
The 0.534 kb PstI fragment detects mRNA of 3.8 and 3.2 kb from mouse mammary tumors.
References

Zimmermann W, et al. Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure. Proc. Natl. Acad. Sci. USA 84: 2960-2964, 1987. PubMed: 3033671

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