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pBS-hTOP2 [UTR-03525, WI-7251]

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價格:

編號:B239700

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pBS-hTOP2 [UTR-03525, WI-7251]

商品貨號

B239700

Designations

pBS-hTOP2 [UTR-03525, WI-7251]

GenBank Number

J04088

培養基

Species

Homo sapiens, human

Depositors

JC Wang

Vector

Construct size (kb): 8.600000381469727

Insert

DNA: cDNA

Insert lengths(kb): 5.599999904632568

Gene product: DNA topoisomerase II, alpha, 170kDa( DNA Topoisomerase II alpha, DNA topoisomerase II, 170 kD, topoisomerase (DNA) II alpha (170kD)) [TOP2]

Insert Size (kb)

5.600

Media

ATCC^® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: Frozen

Comments

Restriction digests of the clone give the following sizes (kb): MluI/XhoI--5.6, 3.0; EcoRI--4.3, 3.2, 1.1; ClaI--8.6; HindIII--4.4, 4.2.

The insert contains the following restriction sites (approximate kb from the 5' MluI site): BamHI--0.74, 3.25, 4.27; EcoRI--1.3, 4.3; HindIII--1.5; BglII--2.14, 5.2.

Contains the complete cDNA. Constructed from lambdahTOP2-37, lambdahTOP2-11, and lambdahTOP2-Z2, joined at the BglII sites. An MluI linker was added to the filled-in NcoI site at the 5' end,

and a MluI linker was inserted into the SmaI site of the vector.

The original clones were derived from a HeLa cell library (the 2 5' clones) and a HepG2 library (the 3' clone).

References

Tsai-Pflugfelder M, et al. Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22. Proc. Natl. Acad. Sci. USA 85: 7177-7181, 1988. PubMed: 2845399

James C Wang, personal communication

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