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pBKV (35-1)
pBKV (35-1)
規(guī)格:
價(jià)格:
編號(hào):B240203
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBKV (35-1)
商品貨號(hào)
B240203
Designations
pBKV (35-1)
GenBank Number

J02039

培養(yǎng)基
Species
BK polyomavirus, BK Virus
Depositors
PM Howley
Vector
Construct size (kb): 9.30
Name of construct: pBKV (35-1)
Excise insert: BamHI
Size of construct (kb): 9.3
Markers: ampR
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
Genome: BK virus
Contains complete coding sequence?: U
Strain: MM
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: J02039
Insert Size (kb)
4.963
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
SPECIAL CHARACTERISTICS:
ORIGINAL SOURCE: Lysate of infected tissue culture cells. Entire genomic DNA linearized at BamHI site into BamHI site of pBR322
SPECIAL CHARACTERISTICS:
The entire viral genome was isolated from an infected human brain. BK virus was linearized with BamHI before cloning.
Classification
Polyomaviridae, Polyomavirus
References

Yang RC, Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science 206: 456-462, 1979. PubMed: 228391

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