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Hf677 [pHF677]
Hf677 [pHF677]
規(guī)格:
價(jià)格:
編號(hào):B240426
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Hf677 [pHF677]
商品貨號(hào)
B240426
Designations
Hf677 [pHF677]
GenBank Number

K01228

培養(yǎng)基
Species
Homo sapiens, human
Depositors
M Chu
Vector
Construct size (kb): 6.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.799999952316284
Tissue: human fibroblast
Gene product: collagen, type I, alpha 1 [COL1A1]
Insert Size (kb)
1.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.5, 0.3; HindIII--6.2; PstI--6.2; BamHI--6.2; PstI/BamHI--3.0, 2.7.
The insert contains the following restriction sites (number): AvaI (2), HinfI (3), TaqI (6), XhoI (1).
The insert encodes amino acids 787 to the carboxy terminus, and includes 270 nucleotides 3' untranslated.
The insert detects the genomic HindIII fragments of the following sizes (kb): human--6.0; Chinese hamster--8.8, 2.8; mouse--2.7, 2.0.
The probe detects genomic EcoRI fragments of 19.0 and 5.4 kb in the human, 11.5 kb in the rat, and 14.0 kb in the mouse.
The probe detects mRNA of 5.8 and 4.8 kb in human fibroblasts.
References

Chu ML, et al. Cloning and characterization of five overlapping cDNAs specific for the human pro alpha1(I) collagen chain. Nucleic Acids Res. 10: 5925-5934, 1982. PubMed: 6183642

Huerre C, et al. Human type I procollagen genes are located on different chromosomes. Proc. Natl. Acad. Sci. USA 79: 6627-6630, 1982. PubMed: 6292910

Leach RJ, et al. Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics 5: 167-176, 1989. PubMed: 2571568

Bernard MP, et al. Nucleotide sequences of complementary deoxyribonucleic acids for the proalpha1 chain of human type I procollagen. Statistical evaluation of structures that are conserved during evolution. Biochemistry 22: 5213-5223, 1983. PubMed: 6689127

Mon-Li Chu, personal communication

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