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BP-TIE2-R2-1

規格:

價格:

編號:B241578

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

BP-TIE2-R2-1

商品貨號

B241578

Designations

BP-TIE2-R2-1

Species

Homo sapiens, human

培養基

Depositors

R Haronen

Vector

Construct size (kb): 8.899999618530273

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBacPAK8
Intact vector size: 5.538
Type of vector: phagemid
Vector end: XhoI
Vector end: EagI
Cloning sites: BstXI mLuI EcoRV HindIII DraIII ScaI
Polylinker sites: BamHI Sse8387I PstI StuI XhoI BstBI XbaI BglII Asp718I KpnI
Ecl136II SacI EcoRI XmaI SmaI EagI NotI PacI
Features (with orientation and position when available):
promoter: polyhedrin, ->
MCS: multiple cloning site, ->
stop codons: stop codons, ->
marker(s): ampR
replicon: M13
replicon: pMB1
coding sequence: AcMNPV
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TEK
Gene name: TEK tyrosine kinase, endothelial, mutated
ORF's seq. position: DNA Seq. Acc.: L06139 ()
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p21
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EagI
Insert size (kb): 3.4
Cross references: GDB probe: GDB:344185

Insert lengths(kb): 3.400000095367432

Gene product: TEK tyrosine kinase, endothelial, mutated [TEK]

Insert Size (kb)

3.400

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--7.7, 1.2; NcoI/XhoI--5.5, 2.8, 0.7.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.9, 1.4; SmaI--2.9.

ATCC 87508 contains wild-type receptor tyrosine kinase TIE2 and ATCC 87507 contains a mutant gene that has a point mutation at position 2545 from C to T, resulting in an R849W substitution.

References

Vikkula M, et al. Vascular dysmorphogenesis caused by and activating mutation in the receptor tyrosine kinase TIE2. Cell 87: 1181-1190, 1996. PubMed: 8980225

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