Restriction digests of the clone give the following sizes (kb): SmaI--10.3; HindIII--8.0, 2.1; EcoRI--4.5, 3.15, 2.25.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
The vector was constructed by Lenny Guarente.
Growth Conditions
Temperature: 37.0°C
References
Guarente L, Hoar E. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA" box. Proc. Natl. Acad. Sci. USA 81: 7860-7864, 1984. PubMed: 6096863
