contains easily purifiable cassette(s) for construction omega-aadA1
Comments
Restriction digests of the clone give the following sizes (kb): MluI--2.2, 2.1; EcoRI--2.2, 2.1; BamHI--2.2, 2.1.
Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation.
Media
ATCC® Medium 1632: LB medium (ATCC medium 1065) with 10 mcg/ml chloramphenicol
Growth Conditions
Temperature: 37.0°C
References
Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
