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pSP417

規(guī)格:

價(jià)格:

編號(hào):B243665

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pSP417

商品貨號(hào)

B243665

Designations

pSP417

Depositors

SM Podkovyrov

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 9.1999998092651370

Vector: pSP417 (plasmid)

Construction: pRS415

Marker(s):ampR

Construct size (kb): 9.199999809265137

Features: marker(s): ampR
replicon: pMB1
reporter group: lacZ
MCS: EcoRI...BamHI
transcription terminator: T1
coding sequence: lacY

Applications

promoter-cloning vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--9.2; BglII--9.2; SalI--9.2; NotI--9.2; PvuI--6.2, 1.8, 0.75, 0.45.

Distributed in E. coli strain SP2 (ATCC 47089) which contains a pcnB mutation that limits pMB1-based plasmid copy number to approximately 10% of wild type. This is useful where high copy number may interfere with the fusion analysis.

A high copy number, promoter-cloning plasmid that with very low levels of background lacZ expression. Permits detection of weak transcription signals.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Simons RW, et al. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96, 1987. PubMed: 3596251

Podkovyrov SM, Larson TJ. A new vector-host system for construction of lacZ transcriptional fusions where only low-level gene expression is desirable. Gene 156: 151-152, 1995. PubMed: 7737510

Shipped

freeze-dried

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