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pUNG40

規格:

價格:

編號:B243757

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUNG40

商品貨號

B243757

Designations

pUNG40

GenBank Number

X15653

培養基

Species

Homo sapiens, human

Applications

It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases.

Vector

Construct size (kb): 4.735000133514404

Insert

DNA: cDNA

Insert lengths(kb): 1.726999998092651

Tissue: placenta

Gene product: uracil-DNA glycosylase(uracil DNA glycosylase) [DGU]

Insert Size (kb)

1.727

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.5, 0.2; EcoRI--3.0, 1.7; XbaI--4.7; HindIII--3.8, 0.9; XhoI--4.7.

The insert contains the following restriction sites (nt from 5' end[nt according to published sequence]): BamHI--15, 256 [297, 538]; SacII--33 [315]; StuI--308 [590]; NcoI--388 [670]; HindIII--850 [1132].

Gives a strong hybridization signal with genomic EcoRI fragments of 7 and 3.5 kb.

Extends from nt 283 to nt 2005 of the published sequence. It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases.

Oriented such that transcription from the T7 promoter gives the sense strand.

References

Olsen LC, et al. Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme*. EMBO J. 8: 3121-3125, 1989. PubMed: 2555154

Aasland R, et al. Chromosomal assignment of human uracil-DNA glycosylase to chromosome 12. Genomics 7: 139-141, 1990. PubMed: 2335354

Lisbeth C Olsen, personal communication

B210768：pUNG40

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