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HFBEF62

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價格:

編號:B243794

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

HFBEF62

商品貨號

B243794

Designations

HFBEF62

GenBank Number

T07227

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 3.75

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: UCHL1
Gene name: ubiquitin carboxyl-terminal esterase L1
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 p14
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.76
Cross references: DNA Seq. Acc.: T07227; EST: EST05116; Primer: M13-21; THC
id: THC100992
DNA Seq. Acc.: X04741; EST: EST05116
Nucleotides 23-279, 275-330 of the insert correspond to
nucleotides 985-729, 734-679 of X04741 with 97% identity.

Insert lengths(kb): 0.7599999904632568

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: ubiquitin carboxyl-terminal esterase L1(ubiquitin carboxyl-terminal hydrolase PGP9.5) [UCHL1]

Insert Size (kb)

0.760

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.41, 0.35.

The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs.

References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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