Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells are free from the classical swine fever viruses and porcine circoviruses as described in 9 CFR 113.46, 9 CFR 113.47 and 9 CFR 113.55.
The SK-RST cells exhibited cytopathology after being infected with two serotypes of vesicular stomatitis virus, Newcastle disease virus, porcine parvovirus, pseudorabies virus, reovirus, transmissible gastroenteritis virus, bovine virus diarrhea, bovine adenovirus, bovine parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and with representatives of the A, O, C, SAT1, SAT2, SAT3 and Asia serotypes of foot-and-mouth disease virus.
Classical swine fever virus (CSF)-infected SK-RST cells produced CSF antigen that was detectable using a CSF-antigen specific monoclonal antibody in an avidin-biotin complex immunohistochemistry assay.
The SK-RST cells failed to exhibit cytopathology when exposed to porcine adenovirus, bluetongue virus, bovine respiratory syncytial virus, and bovine parvovirus.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
