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pSV2RbetaG [pSV2beta-globin]

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價格:

編號:B244433

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSV2RbetaG [pSV2beta-globin]

商品貨號

B244433

Designations

pSV2RbetaG [pSV2beta-globin]

Depositors

P Berg, M Dieckmann

培養基

Biosafety Level

Vector Information

Size (kb): 4.7199997901916500

Vector: pSV2RbetaG (plasmid)

Promoters: Promoter SV40 early

Marker(s):ampR

Construct size (kb): 4.71999979019165

Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early

Applications

expression vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/BglII--4.23, 0.49; PvuII/PstI--2.22, l.55, 0.96; PstI--3.76, 0.96.

Expresses beta-globin in mammalian cells.

This plasmid includes the SV40 promoter (SV nt 270-5171), rabbit beta globin (487 bp), the SV40 splice site (SV nt4100-4710), the SV40 poly(A) (SV nt 1782-2770) and pBR322 (pBR nt 2067-4362).

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Paul Berg, personal communication

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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