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HTLV-II, deltaH6H11 in pBR322, purified plasmid DNA
HTLV-II, deltaH6H11 in pBR322, purified plasmid DNA
規(guī)格:
價格:
編號:B244947
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HTLV-II, deltaH6H11 in pBR322, purified plasmid DNA
商品貨號
B244947
Designations
HTLV-II, deltaH6H11 in pBR322, purified plasmid DNA
GenBank Number

M10060

培養(yǎng)基
Species
human T-cell leukemia virus II
Depositors
IS Chen
Applications
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
Vector
Construct size (kb): 13.2
Insert
DNA: genomic
Insert lengths(kb): 10.10000038146973
Insert Size (kb)
10.100
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells.
The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4.
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb.
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.0 (doublet), 0.92, 0.36; HindIII--10.5, 3.2; XbaI--13.5.
References

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323

Irvin S Chen, personal communication

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