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JAR
JAR
規(guī)格:
價格:
編號:B257530
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
JAR
商品貨號
B257530
<strong>Cell line </strong>
JAR
DSMZ no.
ACC 462
培養(yǎng)基
Species
confirmed as human by cytogenetics
Cell type
choriocarcinoma
Origin
established from the trophoblastic tumor of the placenta of a 24-year-old Caucasian woman, but the cell line is from the male fetus
Reference(s)
15154
Biosafety level
1
Permissions and restrictions
A
<strong>&nbsp;</strong>
DSMZ Scientific Data:
Morphology
epithelial-like cells growing adherent in monolayers and in clusters
Medium
85% RPMI 1640 + 15% h.i. FBS
Subculture
split culture 1:5 to 1:10 every 3-4 days before reaching confluency using trypsin/EDTA; seed out at ca. 3-4 x 106 cells/80 cm2; confluent cultures tend to form aggregates, that loosely adhere to the culture vessel surface; however, these aggregates are difficult to disperse and may need trypsin treatment after a centrifugation and washing step
Incubation
at 37 °C with 5% CO2
Doubling time
about 20-30 hours
Harvest
cell harvest of ca. 10-15 x 106 cells/80 cm2
Storage
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma
contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint
multiplex PCR of minisatellite markers revealed a unique DNA profile
Cytogenetics
human flat-moded near-triploid karyotype with 27% polyploidy - 60-70<3n>XXY, +1, -2, -5, -7, -8, -9, -13, -13, -14, -15, -17, -19, -21,+12mar, +acf, add(X)(p22), del(1)(p3?p3?), add(1)(q11), der(1)add(1)(p11)add(1)(q4?), inv(3)(p25.2q24), add(3)(q13), add(4)(q?31), del(4)(q2?2), add(9)(p21), add(12)(q24), i(13)(q10)dup(13)(q11q34), add(16)(q24), add(17)(p12), add(18)(p11.2) - sideline with add(2)(p2?), del(8)(q22), add(21)(p11) - resembles published karyotype
Viruses
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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