Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Animals were immunized with human lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody has conventional CD45 reactivity and is included as antibody 815 in the panel used in the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens.
The antibody has a higher affinity for CD45 than the BC8 antibody (see ATCC HB-10507).
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).Matthews DC, et al. Radiation delivery to lymphoid and marrow tissues. US Patent 5,273,738 dated Dec 28 1993
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
