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B2-1
B2-1
規格:
價格:
編號:B163976
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B2-1
商品貨號
B163976
Organism
Mus musculus, mouse
Tissue
embryo
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
embryo
Strain
BALB/c
Applications
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Tumorigenic
No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR.
Complete Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with: 0.05 mg/ml 5-iodo-2'-deoxyuridine 10% heat-inactivated fetal bovine serum
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Remove IUdR for one passage before using.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
The United States of America
U.S. Patent Number
References

Hampar B, Showalter SD. Non-transformed thymidine kinaseless cell line and its use for testing tumorigenic potential of genes. US Patent 4,465,769 dated Aug 14 1984

Hampar B, et al. Herpes simplex virus (type 1) thymidine kinase gene does not transform cells morphologically. Proc. Natl. Acad. Sci. USA 78: 2616-2619, 1981. PubMed: 6264486

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