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當(dāng)前位置: 首頁 > ATCC代理 > C44 B164094
C44
C44
規(guī)格:
價(jià)格:
編號:B164094
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
C44
商品貨號
B164094
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds with high affinity to colchicine, and with lower affinity to related compounds.
It is useful for monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity.
Derivation
The C44 hybridoma was originated by S.K. Edmond Rouan in 1987.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against colchicine
Cellular Products
immunoglobulin; monoclonal antibody; against colchicine
Comments
The C44 hybridoma was originated by S.K. Edmond Rouan in 1987.
Animals were immunized with a colchicine - keyhole limpet hemocyanin (KLH) conjugate.
Spleen cells were fused with NS-1 myeloma cells.
The antibody binds with high affinity to colchicine, and with lower affinity to related compounds.
The antibody is bound strongly by Protein A.
It is useful for monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
AC Cunningham
Deposited As
mouse (B cell); mouse (myeloma)
References

Edmond Rouan SK, et al. Specific, high affinity colchicine binding monoclonal antibodies: development and characterization of the antibodies. Hybridoma 8: 435-448, 1989. PubMed: 2777275

Rouan SK, et al. Reversal of colchicine-induced mitotic arrest in Chinese hamster cells with a colchicine-specific monoclonal antibody. Am. J. Pathol. 137: 779-787, 1990. PubMed: 2221012

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