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CHL-1

規(guī)格:

價格:

編號:B164248

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

CHL-1

商品貨號

B164248

Organism

Homo sapiens, human

Tissue

skin

培養(yǎng)基

Cell Type

melanoma

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

melanoma

Applications

Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 10⁷ cells/mL.

Storage Conditions

liquid nitrogen vapor temperature

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This is a derivative of the RPMI 7932 human melanoma cell line (Bowes).

Genes Expressed

human tissue plasminogen activator (t-PA)

Cellular Products

human tissue plasminogen activator (t-PA)

Comments

This cell line produces about 0.1 mU of t-PA per cell per day.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Add fresh medium at the time of subculture

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10

D13S317: 8,11

D16S539: 13

D5S818: 11,12

D7S820: 11,13

THO1: 9,9.3

TPOX: 8

vWA: 17

Name of Depositor

Berlex Laboratories, Inc.

U.S. Patent Number

5,017,478

References

Cashion L, et al. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991

B164247：CHL/IU [CHL-11]

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