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IGEL a2 [15.3]
IGEL a2 [15.3]
規格:
價格:
編號:B164834
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IGEL a2 [15.3]
商品貨號
B164834
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor temperature
Genes Expressed
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Cellular Products
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Comments
Animals were immunized with TNP-Asc.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype
IgE (Iga haplotype)
Name of Depositor
M Wabl
Deposited As
mouse (B cell); mouse (myeloma)
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Cross References

Nucleotide (GenBank) : X65774 M.musculus DNA for IgE antibody light chain (VJ).

Nucleotide (GenBank) : X65772 M.musculus DNA for IgE antibody heavy chain (VDJ).

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