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J.gamma1
J.gamma1
規格:
價格:
編號:B164867
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
J.gamma1
商品貨號
B164867
Organism
Homo sapiens, human
Product Format
frozen
培養基
Morphology
lymphoblast
Culture Properties
clusters in suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute T cell leukemia
Gender
male
Derivation
Wild-type Jurkat cells were mutagenized with the frameshifting mutagen ICR-191 and clones were isolated that failed to increase calcium in response to pervanadate. The selection procedure was repeated on the P98 clone to establish the J.gamma1 cell line.
Clinical Data
male
Comments

The J.gamm1 cell line is a phospholipase C-gamma1 (PLC-gamma1) deficient mutant of the E6-1 clone of Jurkat (ATCC TIB-152).

The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.

The J.gamma1 subline contains no detectable PLC-gamma1 protein. The lack of PLC-gamma1 expression in J.gamma1 cells causes profound defects in T cell receptor (TCR) calcium mobilization and nuclear factor of activated T-cells (NFAT) activation.

These transcriptional defects are reversed by transfection of P98 cells with a wild-type PLC-gamma1 expression vector.



Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL.
Maintain cell density between 1 X 105 and 2 to 3 X 106 viable cells/mL.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19
Name of Depositor
RT Abraham
Deposited As
human
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067

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