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KG-1

規格:

價格:

編號:B164908

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

KG-1

商品貨號

B164908

Organism

Homo sapiens, human

Tissue

bone marrow

培養基

Cell Type

Macrophage

Product Format

frozen

Morphology

myeloblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

acute myelogenous leukemia

Age

59 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations.

Derivation

The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia.

Clinical Data

59 years

Caucasian

male

Antigen Expression

HLA A30, A31, B35, Cw4

Genes Expressed

HLA DR

Cellular Products

HLA DR

Comments

KG-1 myeloblasts can differentiate into macrophages in the presence of phorbol esters.

They show a good response to colony stimulating factor (CSF).

The line is EBNA negative (EBNA-).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cell density between 2 x 10⁵ and 1 x 10⁶ viable cells/mL. Do not allow to exceed 2 x 10⁶ cells/mL.

Medium Renewal: Twice per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 7

D13S317: 11,12

D16S539: 10,11

D5S818: 13

D7S820: 8,10

THO1: 7,8

TPOX: 7,9

vWA: 14,19

Isoenzymes

AK-1, 0

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1-2

PGM3, 0

Name of Depositor

DW Golde

Deposited As

Homo sapiens

References

Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689

Hester JP et al. Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel. Blood 54(1): 254-268 1979. PubMed: 444670

Cross References

Nucleotide (GenBank) : AF146432 Homo sapiens cell-line KG-1a sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

B164910：KG-1a

B164909：KG-1

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