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KG-1
KG-1
規格:
價格:
編號:B164909
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
KG-1
商品貨號
B164909
Organism
Homo sapiens, human
Product Format
frozen
培養基
Morphology
lymphoblast-like
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute myelogenous leukemia
Age
59 years
Gender
male
Ethnicity
Caucasian
Applications
Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Caucasian
59 years
Genes Expressed
human leukocyte interferon
Cellular Products
human leukocyte interferon
Comments
Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
See also ATCC CCL-246.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Name of Depositor
Genentech, Inc.
U.S. Patent Number
References

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987

Cross References

Nucleotide (GenBank) : E00473 Human leukocyte interferon A (LeIFA) gene and its flanking sequence.

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