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MH-S
MH-S
規格:
價格:
編號:B165152
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MH-S
商品貨號
B165152
Organism
Mus musculus, mouse
Tissue
lung
培養基
Cell Type
macrophage (alveolar)
Product Format
frozen
Morphology
macrophage
Culture Properties
mixed, adherent and suspension
Biosafety Level
2    [Cells may contain SV40 virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
7 weeks
Gender
male
Strain
BALB/c
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
The MH-S cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages.
Clinical Data
male
7 weeks
Antigen Expression
CD11b (Mac-1); Class II antigens (I-A); T antigen
Receptor Expression
Fc

Genes Expressed
interleukin 1 (IL-1)
Cellular Products
interleukin 1 (IL-1)
Comments
The cells retain many of the properties of alveolar macrophages including typical macrophage morphology.
They are adherent, phagocytic, esterase positive and peroxidase negative.
Lipopolysaccharide (LPS) treatment stimulates IL-1 production.
The cells are capable of suppressing the in vitro plaque forming cell (PFC) response in a cell dose dependent manner.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by transferring floating cells to a centrifuge tube Rinse adherent cells with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add to floating cells collected above and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37°C
Name of Depositor
I Mbawuike, HB Herscowitz
Deposited As
Mus musculus
References

Mbawuike IN, Hercowitz HB. MH-S, a murine alveolar macrophage cell line: morphological, cytochemical, and functional characteristics. J. Leukocyte Biol. 46: 119-127, 1989. PubMed: 2787372

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