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Sp2/0-Ag14
Sp2/0-Ag14
規(guī)格:
價格:
編號:B165748
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Sp2/0-Ag14
商品貨號
B165748
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
Spleen
培養(yǎng)基
Cell Type
Hybridoma: B lymphocyte
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c
Applications
This cell line is a fusion partner for production of somatic cell hybrids.
Storage Conditions
Liquid nitrogen vapor phase
Images
Derivation
The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma.
Antigen Expression
Antigen expression: H-2d
Comments
The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 μg/ml and are HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Initiate the cells at a start-up seeding density of 1.0 x 10e5 viable cells/mL. Maintain cell density between 1 X 10e4 and 1 X 10e6 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor
G Kohler
Deposited As
mouse (B cell); mouse (myeloma)
References

Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293

Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022

Cross References

Nucleotide (GenBank) : NC_001702 Murine type C retrovirus, complete genome.

Nucleotide (GenBank) : X94150 Retroviridae complete genome (murine type C retrovirus).

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