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Y-190 [Y190]
Y-190 [Y190]
規(guī)格:
價格:
編號:B190751
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Y-190 [Y190]
商品貨號
B190751
Designations
Y-190 [Y190]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
U Muller, Institute of Human Genetics
Applications
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
Vector
Construct size (kb): 7.90
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
Insert lengths(kb): 3.5
Genomic copy number: repetitive
Gene name: DNA Segment, repetitive
Contains complete coding sequence?: U
Chromosome: Y; Localization: Y p
Tissue: 49,XXXXY cell line
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Genome: Homo sapiens
Gene symbol: DYZ5
Type of nucleic acid: genomic
Restriction enzyme: XbaI
Number of alleles: >5
Size range (kb): 550-700
Type of polymorphism: VNTR
Insert Size (kb)
3.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.5; EcoRI--6.1, 1.8; AvaI--4.7, 3.2; BamHI--8.0; PvuII--8.0.
The sequences detected by Y-190 are present in numerous XX males.
This probe hybridizes to Y-specific HindIII fragments of about 4 and 5 kb (strong) and 9 kb (weak) in the orangutan and 4 kb and 9 kb (weak) in the rhesus monkey.
This probe detects 2 blocks of repeats on Yp. The hypervariable major block is composed of tandem 20.3 kb repeats with alleles ranging from about 540 to 800 kb (XbaI). The minor block is not variable and is about 60 kb.
Detects a Y-specific 3.5 kb HindIII fragment that has a copy number of about 100 in normal human males. Also detects less repeated HindIII fragments ranging from 1.5 to 5.7 kb.
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
References

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

Muller U, et al. Y-190, a DNA probe for the sensitive detection of Y-derived marker chromosomes and mosaicism. Hum. Genet. 75: 109-113, 1987. PubMed: 3469135

Tyler-Smith C, et al. Structure of a hypervariable tandemly repeated DNA sequence on the short arm of the human Y chromosome. J. Mol. Biol. 203: 837-848, 1988. PubMed: 3210241

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