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Y-190 [Y190]
Y-190 [Y190]
規格:
價格:
編號:B237286
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Y-190 [Y190]
商品貨號
B237286
Designations
Y-190 [Y190]
Species
Homo sapiens, human
培養基
Applications
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
Vector
Construct size (kb): 7.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 3.5
Tissue: 49,XXXXY cell line
Gene product: DNA Segment, repetitive [DYZ5]
Insert Size (kb)
3.5
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.5; EcoRI--6.1, 1.8; AvaI--4.7, 3.2; BamHI--8.0; PvuII--8.0.
The sequences detected by Y-190 are present in numerous XX males.
This probe hybridizes to Y-specific HindIII fragments of about 4 and 5 kb (strong) and 9 kb (weak) in the orangutan and 4 kb and 9 kb (weak) in the rhesus monkey.
This probe detects 2 blocks of repeats on Yp. The hypervariable major block is composed of tandem 20.3 kb repeats with alleles ranging from about 540 to 800 kb (XbaI). The minor block is not variable and is about 60 kb.
Detects a Y-specific 3.5 kb HindIII fragment that has a copy number of about 100 in normal human males. Also detects less repeated HindIII fragments ranging from 1.5 to 5.7 kb.
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
References

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

Muller U, et al. Y-190, a DNA probe for the sensitive detection of Y-derived marker chromosomes and mosaicism. Hum. Genet. 75: 109-113, 1987. PubMed: 3469135

Tyler-Smith C, et al. Structure of a hypervariable tandemly repeated DNA sequence on the short arm of the human Y chromosome. J. Mol. Biol. 203: 837-848, 1988. PubMed: 3210241

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