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p426 GAL1 plasmid in E. coli

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編號:B191586

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

p426 GAL1 plasmid in E. coli

商品貨號

B191586

Designations

p426 GAL1 plasmid in E. coli

Depositors

M Funk

培養基

Biosafety Level

Vector Information

Size (kb): 6.417

Vector: p426 GAL1 (plasmid)

Promoters: Promoter for expression GAL1

Construction: pRS426 (ATCC 77107), GAL1 promoter

Marker(s):URA3,ampR

Construct size (kb): 6.417

Features: marker(s): URA3
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...XpeI
terminator: CYC1

Applications

YE-type (episomal) shuttle vector

YX-type (expression) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.5; EcoRI--6.4; KpnI--6.4.

Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.

High copy number shuttle expression vector.

One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736