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p426 GALS
p426 GALS
規(guī)格:
價(jià)格:
編號(hào):B232180
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p426 GALS
商品貨號(hào)
B232180
Designations
p426 GALS
Depositors
M Funk
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 6.3720002174377440
Vector: p426 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS426 (ATCC 77107), GALS promoter
Marker(s):URA3,ampR
Construct size (kb): 6.372000217437744
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GALS
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; SacI/XbaI--3.4, 2.9, 0.48.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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