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pSH

規格:

價格:

編號:B228481

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSH

商品貨號

B228481

Designations

pSH

Depositors

AM Ventura

培養基

Biosafety Level

Vector Information

Size (kb): 5.9000000953674320

Vector: pSH (plasmid)

Promoters: Promoter T3

Construction: pEN1, BLpSV

Marker(s):ampR

Construct size (kb): 5.900000095367432

Features: marker(s): ampR
promoter: HTLV-1 LTR, T7, T3
replicon: pMB1, SV40
terminator: SV40 polyadenylation
enhancer: HIV-1 LTR

Applications

expression vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; HindIII--5.9; PstI--4.3, 1.6.

Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse cells.

The order of the major features in the plasmid is: T7 promoter - XhoI - SV40 promoter - HTLV-1 LTR promoter - HIV-1 LTR enhancer - EcoRI/MCS/BglII - SV40 polyadenylation - T3 promoter - pMB1 ori - ampR.

Media

ATCC^® Medium 1946: Terrific broth (ATCC medium 1743) with 100 mcg/ml ampicillin

Growth Conditions

Temperature: 30.0°C

References

Ventura AM, Villa LL. Plasmid pSH: a strong mammalian expression vector. Biochem. Biophys. Res. Commun. 192: 867-869, 1993. PubMed: 8387291

Shipped

freeze-dried

Shipping Information

Freeze dried plasmid in E. coli

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