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delta TA4-1 [dTA4-1]
delta TA4-1 [dTA4-1]
規格:
價格:
編號:B164345
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
delta TA4-1 [dTA4-1]
商品貨號
B164345
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human delta heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human delta heavy chain
Comments
Animals were immunized with human delta heavy chain.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG3; kappa light chain
Name of Depositor
JF Kearney, H Kubagawa
Deposited As
mouse (B cell); mouse (myeloma)
References

. . J. Clin. Immunol. 2: 942-945, 1982.

Kubagawa H, et al. Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J. Clin. Immunol. 2: 264-269, 1982. PubMed: 6815220

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